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121st may refer to:

• 121st (Western Irish) Battalion, CEF, a unit in the Canadian Expeditionary Force during the First World War
• 121st Air Refueling Wing (121 ARW) is an aerial refueling organization assigned to the Ohio Air National Guard
• 121st Engineer Battalion, one of the first American units to land in Normandy on D-Day during World War II
• 121st Fighter Squadron flies the F-16C/D Fighting Falcon
• 121st Infantry Division (German Empire), a unit of the Imperial German Army in World War I
• 121st International Olympic Committee Session, 2009-10-02 in Denmark, when the 2016 Summer Olympics location will be decided
• 121st meridian east, 121° east of Greenwich, a line of longitude through Asia, the Indian Ocean and Australia
• 121st meridian west, 121° west of Greenwich, a line of longitude through North America and the Pacific Ocean
• 121st New York Volunteer Infantry Regiment, a volunteer regiment recruited during the American Civil War
• 121st Pennsylvania Regiment, a regiment of the Union Army during the United States Civil War
• 121st Regiment of Foot (1762), an infantry regiment of the British Army, formed in 1762 and disbanded in 1764
• 121st Regiment of Foot (1794), an infantry regiment of the British Army, formed in 1794 and disbanded in 1796
• 121st Signal Battalion (United States), a signal unit of the United States Army, inactivated as of July 2006
• 121st Street (BMT Jamaica Line), a station on the elevated BMT Jamaica Line of the New York City Subway
• 121st Street (IRT Second Avenue Line), a station on the demolished IRT Second Avenue Line
• 121st Weather Flight, a subordinate unit of the 113th Wing of the United States Air Force based at Andrews Air Force Base, Maryland

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