In eukaryotes, it is synthesised by RNA
polymerase III (the other eukaryotic rRNAs are cleaved from a
45S precursor synthesized by RNA polymerase I). In Xenopusoocytes, it has been shown that fingers 4-7 of
the nine-zinc fingertranscription factor TFIIIA can
bind to the central region of 5S RNA.
Binding between 5S rRNA and TFIIIA serves to both repress further
transcription of the 5S RNA gene and stabilize the 5S RNA
transcript until it is required for ribosome assembly.
^Mueller F, Sommer I, Baranov P, Matadeen
R, Stoldt M, Wöhnert J, Görlach M, van Heel M, Brimacombe R (2000).
"The 3D arrangement of the 23 S and 5 S rRNA in the Escherichia
coli 50 S ribosomal subunit based on a cryo-electron microscopic
reconstruction at 7.5 A resolution.". J Mol Biol298 (1): 35-59. PMID10756104.
^Mitra K, Schaffitzel C, Fabiola F, Chapman
MS, Ban N, Frank J (2006). "Elongation arrest by SecM via a cascade
of ribosomal RNA rearrangements.". Mol Cell22 (4): 533-43. PMID16713583.