AFL is a three-letter acronym that may mean one of the following:

Sports leagues

• Australian Football League, an Australian rules football competition in Australia
• Austrian Football League, an American football league in Austria
• Arena Football League, an American indoor football league
• American Football League (AFL), name shared by several separate and unrelated professional football leagues:
  • American Football League (1926) (a.k.a "AFL I") - first rival of the National Football League (NFL) that competed in 1926
  • American Football League (1936) (a.k.a "AFL II") - second rival of the NFL that competed in 1936 and 1937
  • American Football League (1938) - minor professional American football league that changed its name to the American Professional Football Association in 1939
  • American Football League (1940) (a.k.a "AFL III") - third rival of the NFL that competed in 1940 and 1941
  • American Football League (1944) - minor professional American football league that merged with the Pacific Coast Professional Football League in 1945
  • American Football League (1946) - name adopted by American Association minor American football league in 1946
  • American Football League (1960) (a.k.a. "AFL IV") - fifth rival of the NFL that started competition in 1960 and merged with the more established league in 1967.
• Alberta Football League, an amateur men's Canadian football league
• Alliance Football League, a semi-professional American football league
• Alberton Football League, competition in Australian rules football
• Anti-Football League, an organisation that bemoans the cultural saturation of the Australian Football League
• Arizona Fall League, American baseball league
• American Fencing League, a league for amateur standard fencing competition in the United States

Labor unions

• American Federation of Labor
• Alberta Federation of Labour

Other

• Abstract family of languages
• Academic Free License
• Adaptive forward lighting
• After fader listening, a function on an audio mixing console
• Aflac New York Stock Exchange abbreviation for, an American insurance company
• Alta Floresta, Brazil IATA airport code
• Ankara Fen Lisesi, more widely known as Ankara Science High School
• Armed Forces of Liberia
• Assessment for Learning
• Autofocus lock, a function that locks the metered focus of a camera
• Automotive Finance & Leasing (UK) Ltd is a Business Contract Hire company and Broker registered in England and Wales (formerly named Autodex Fleet Leasing Ltd)