The Full Wiki

Adenomatosis polyposis coli: Wikis

Advertisements
  

Note: Many of our articles have direct quotes from sources you can cite, within the Wikipedia article! This article doesn't yet, but we're working on it! See more info or our list of citable articles.

Encyclopedia

From Wikipedia, the free encyclopedia

edit
Adenomatosis polyposis coli

PDB rendering based on 1deb.
Available structures
1deb, 1m5i, 1th1, 1v18
Identifiers
Symbols APC; DP2; DP2.5; DP3; FAP; FPC; GS
External IDs OMIM175100 MGI88039 HomoloGene30950 GeneCards: APC Gene
RNA expression pattern
PBB GE APC 216933 x at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 324 11789
Ensembl ENSG00000134982 ENSMUSG00000005871
UniProt P25054 Q8C9I9
RefSeq (mRNA) NM_000038 XM_622559
RefSeq (protein) NP_000029 XP_622559
Location (UCSC) Chr 5:
112.1 - 112.21 Mb
Chr 18:
34.35 - 34.44 Mb
PubMed search [1] [2]

APC (adenomatosis polyposis coli) is a human gene that causes colorectal cancer when it is mutated.[1]

APC is classified as a tumor suppressor gene. Tumor suppressor genes prevent the uncontrolled growth of cells that may result in cancerous tumors. The protein made by the APC gene plays a critical role in several cellular processes that determine whether a cell may develop into a tumor. The APC protein helps control how often a cell divides, how it attaches to other cells within a tissue, or whether a cell moves within or away from a tissue. This protein also helps ensure that the chromosome number in cells produced through cell division is correct. The APC protein accomplishes these tasks mainly through association with other proteins, especially those that are involved in cell attachment and signaling. The activity of one protein in particular, beta-catenin, is controlled by the APC protein (see: Wnt signaling pathway). Regulation of beta-catenin prevents genes that stimulate cell division from being turned on too often and prevents cell overgrowth.

The human APC gene is located on the long (q) arm of chromosome 5 between positions 21 and 22, from base pair 112,118,468 to base pair 112,209,532. The APC gene has been shown to contain an internal ribosome entry site. APC orthologs[2] have also been identified in all mammals for which complete genome data are available.

The full-length human protein comprises 2843 amino acids with a (predicted) molecular mass of 311646 Da. Most domains of this protein are solved structurally and exhibit high intrinsic disorder and flexibility as a monomer, and a low content of stable secondary structure. Thus it is a member of the intrinsically unstructured proteins). Little is known about the in vivo full-length unfolded protein.

Contents

Role in cancer

The most common mutation in colon cancer is inactivation of APC. When APC does not have an inactivating mutation, beta-catinin does. These mutations can be inherited, or arise sporadically, often as the result of mutations in other genes that produce chromosomal instability. A mutation on APC or beta-catinin must be followed by other mutations to become cancerous; however, in carriers of an APC inactivating mutations, the risk of colorectal cancer by age 40 is almost 100%.[1]

Familial adenomatous polyposis (FAP) is caused by mutations in the APC gene. More than 800 mutationsin the APC gene have been identified in families with classic and attenuated types of familial adenomatous polyposis. Most of these mutations cause the production of an APC protein that is abnormally short and nonfunctional. This short protein cannot suppress the cellular overgrowth that leads to the formation of polyps, which can become cancerous. The most common mutation in familial adenomatous polyposis is a deletion of five bases (the building blocks of DNA) in the APC gene. This mutation changes the sequence of amino acids (the building material of proteins) in the resulting APC protein beginning at position 1309.

Another mutation is carried by approximately 6 percent of people of Ashkenazi (eastern and central European) Jewish heritage. This mutation results in the substitution of the amino acid lysine for isoleucine at position 1307 in the APC protein (also written as I1307K or Ile1307Lys). This change was initially thought to be harmless, but has recently been shown to be associated with a 10 to 20 percent increased risk of colon cancer.

Regulation of Proliferation

The (Adenomatosis Polyposis Coli) APC protein normally builds a complex with glycogensynthasekinase 3beta(GSK 3β) and Axin via interactions with the 20AA and SAMP repeats. This complex is then able to bind β- catenins in the cytoplasm, that have dissociated from adherens contacts between cells. With the help of Casein Kinase 1 (CK1) which does the first phosphorylation of β-catenin, there is subsequent phosphorylation by GSK-3β. This targets β-catenin for ubuiquitination and degradation by cellular proteosomes. This prevents it from translocating into the nucleus, where it acts as a transcription factor for proliferation genes. APC is also thought to be targeted to microtubules via the PDZ binding domain, stabilising them. The deactivation of the APC protein can take place after certain chain reactions in the cytoplasm are started, e.g. through the Wnt signals that destroy the conformation of the complex. In the nucleus it complexes with legless/BCL9, TCF, and Pygo and begins function of an RNA polymerase but for oncogenes.

Mutations

Mutations in APC often occur early on in cancers such as colon cancer. Patients with familial adenomatous polyposis (FAP) have germline mutations, with 95% being nonsense/frameshift mutations leading to premature stop codons. 33% of mutations occur between amino acids 1061-1309. In somatic mutations, over 60% occur within a mutation cluster region (1286-1513), causing loss of axin binding sites in all but 1 of the 20AA repeats. Mutations in APC lead to loss of β-catenin regulation, altered cell migration and chromosome instability.

Neurological role

Rosenberg et al. found that APC directs cholinergic synapse assembly between neurons, a finding with implications for autonomic neuropathies, for Alzheimer's disease, for age-related hearing loss, and for some forms of epilepsy and schizophrenia.

Interactions

APC (gene) has been shown to interact with

Overview of signal transduction pathways involved in apoptosis.

See also

References

  1. ^ a b Markowitz SD, Bertagnolli MM (December 2009). "Molecular basis of colorectal cancer". N. Engl. J. Med. 361 (25): 2449–60. doi:10.1056/NEJMra0804588. PMID 20018966.  
  2. ^ "OrthoMaM phylogenetic marker: APC coding sequence". http://www.orthomam.univ-montp2.fr/orthomam/data/cds/detailMarkers/ENSG00000134982_APC.xml.  
  3. ^ Kawasaki, Y; Senda T, Ishidate T, Koyama R, Morishita T, Iwayama Y, Higuchi O, Akiyama T (Aug. 2000). "Asef, a link between the tumor suppressor APC and G-protein signaling". Science 289 (5482): 1194–7. PMID 10947987.  
  4. ^ Nakamura, T; Hamada F, Ishidate T, Anai K, Kawahara K, Toyoshima K, Akiyama T (Jun. 1998). "Axin, an inhibitor of the Wnt signalling pathway, interacts with beta-catenin, GSK-3beta and APC and reduces the beta-catenin level". Genes Cells 3 (6): 395–403. PMID 9734785.  
  5. ^ Kaplan, K B; Burds A A, Swedlow J R, Bekir S S, Sorger P K, Näthke I S (Apr. 2001). "A role for the Adenomatous Polyposis Coli protein in chromosome segregation". Nat. Cell Biol. 3 (4): 429–32. doi:10.1038/35070123. PMID 11283619.  
  6. ^ a b Su, L K; Vogelstein B, Kinzler K W (Dec. 1993). "Association of the APC tumor suppressor protein with catenins". Science 262 (5140): 1734–7. PMID 8259519.  
  7. ^ Kucerová, D; Sloncová E, Tuhácková Z, Vojtechová M, Sovová V (Dec. 2001). "Expression and interaction of different catenins in colorectal carcinoma cells". Int. J. Mol. Med. 8 (6): 695–8. PMID 11712088.  
  8. ^ Tickenbrock, Lara; Kössmeier Katja, Rehmann Holger, Herrmann Christian, Müller Oliver (Mar. 2003). "Differences between the interaction of beta-catenin with non-phosphorylated and single-mimicked phosphorylated 20-amino acid residue repeats of the APC protein". J. Mol. Biol. 327 (2): 359–67. PMID 12628243.  
  9. ^ Davies, G; Jiang W G, Mason M D (Apr. 2001). "The interaction between beta-catenin, GSK3beta and APC after motogen induced cell-cell dissociation, and their involvement in signal transduction pathways in prostate cancer". Int. J. Oncol. 18 (4): 843–7. PMID 11251183.  
  10. ^ Ryo, A; Nakamura M, Wulf G, Liou Y C, Lu K P (Sep. 2001). "Pin1 regulates turnover and subcellular localization of beta-catenin by inhibiting its interaction with APC". Nat. Cell Biol. 3 (9): 793–801. doi:10.1038/ncb0901-793. PMID 11533658.  
  11. ^ a b c Homma, Miwako Kato; Li Dongxia, Krebs Edwin G, Yuasa Yasuhito, Homma Yoshimi (Apr. 2002). "Association and regulation of casein kinase 2 activity by adenomatous polyposis coli protein". Proc. Natl. Acad. Sci. U.S.A. 99 (9): 5959–64. doi:10.1073/pnas.092143199. PMID 11972058.  
  12. ^ Satoh, K; Yanai H, Senda T, Kohu K, Nakamura T, Okumura N, Matsumine A, Kobayashi S, Toyoshima K, Akiyama T (Jun. 1997). "DAP-1, a novel protein that interacts with the guanylate kinase-like domains of hDLG and PSD-95". Genes Cells 2 (6): 415–24. PMID 9286858.  
  13. ^ Eklof Spink, K; Fridman S G, Weis W I (Nov. 2001). "Molecular mechanisms of beta-catenin recognition by adenomatous polyposis coli revealed by the structure of an APC-beta-catenin complex". EMBO J. 20 (22): 6203–12. doi:10.1093/emboj/20.22.6203. PMID 11707392.  
  14. ^ a b Daniel, J M; Reynolds A B (Sep. 1995). "The tyrosine kinase substrate p120cas binds directly to E-cadherin but not to the adenomatous polyposis coli protein or alpha-catenin". Mol. Cell. Biol. 15 (9): 4819–24. PMID 7651399.  
  15. ^ Makino, K; Kuwahara H, Masuko N, Nishiyama Y, Morisaki T, Sasaki J, Nakao M, Kuwano A, Nakata M, Ushio Y, Saya H (May. 1997). "Cloning and characterization of NE-dlg: a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein". Oncogene 14 (20): 2425–33. doi:10.1038/sj.onc.1201087. PMID 9188857.  
  16. ^ Jimbo, Takeshi; Kawasaki Yoshihiro, Koyama Ryo, Sato Rina, Takada Shinji, Haraguchi Keiko, Akiyama Tetsu (Apr. 2002). "Identification of a link between the tumour suppressor APC and the kinesin superfamily". Nat. Cell Biol. 4 (4): 323–7. doi:10.1038/ncb779. PMID 11912492.  
  17. ^ Su, L K; Burrell M, Hill D E, Gyuris J, Brent R, Wiltshire R, Trent J, Vogelstein B, Kinzler K W (Jul. 1995). "APC binds to the novel protein EB1". Cancer Res. 55 (14): 2972–7. PMID 7606712.  
  18. ^ Nakamura, M; Zhou X Z, Lu K P (Jul. 2001). "Critical role for the EB1 and APC interaction in the regulation of microtubule polymerization". Curr. Biol. 11 (13): 1062–7. PMID 11470413.  
  19. ^ Shibata, T; Gotoh M, Ochiai A, Hirohashi S (Aug. 1994). "Association of plakoglobin with APC, a tumor suppressor gene product, and its regulation by tyrosine phosphorylation". Biochem. Biophys. Res. Commun. 203 (1): 519–22. doi:10.1006/bbrc.1994.2213. ISSN 0006-291X. PMID 8074697.  
  20. ^ Liu, J; Stevens J, Rote C A, Yost H J, Hu Y, Neufeld K L, White R L, Matsunami N (May. 2001). "Siah-1 mediates a novel beta-catenin degradation pathway linking p53 to the adenomatous polyposis coli protein". Mol. Cell 7 (5): 927–36. PMID 11389840.  
  21. ^ Li, Qingjie; Dashwood Roderick H (Oct. 2004). "Activator protein 2alpha associates with adenomatous polyposis coli/beta-catenin and Inhibits beta-catenin/T-cell factor transcriptional activity in colorectal cancer cells". J. Biol. Chem. 279 (44): 45669–75. doi:10.1074/jbc.M405025200. PMID 15331612.  
  22. ^ Zumbrunn, J; Kinoshita K, Hyman A A, Näthke I S (Jan. 2001). "Binding of the adenomatous polyposis coli protein to microtubules increases microtubule stability and is regulated by GSK3 beta phosphorylation". Curr. Biol. 11 (1): 44–9. PMID 11166179.  
  23. ^ Tickenbrock, Lara; Cramer Janina, Vetter Ingrid R, Muller Oliver (Aug. 2002). "The coiled coil region (amino acids 129-250) of the tumor suppressor protein adenomatous polyposis coli (APC). Its structure and its interaction with chromosome maintenance region 1 (Crm-1)". J. Biol. Chem. 277 (35): 32332–8. doi:10.1074/jbc.M203990200. PMID 12070164.  

Further reading

External links

Advertisements

Advertisements






Got something to say? Make a comment.
Your name
Your email address
Message