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From Wikipedia, the free encyclopedia

An assay is a procedure in molecular biology for testing and/or measuring the activity of a drug or biochemical in an organism or organic sample.[1][2] A quantitative assay may also measure the amount of a substance in a sample. Bioassays and immunoassays are among the many varieties of specialized biochemical assays. Other assays measure processes such as enzyme activity, antigen capture, stem cell activity, and competitive protein binding.

Contents

Assay varieties

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Cytotoxicity

A cytotoxicity assay measures how toxic a chemical compound is to cells.

DNA

Assays for studying interactions of proteins with DNA include:

Protein

RNA

Cell counting

A cell-counting assay, may determine the number of living cells, the number of dead cells, or the ratio of one cell type to another. One example of a cell-counting assay is a blood cell count.

Petrochemistry

Virology

The HPCE-based viral titer assay uses a proprietary, high-performance capillary electrophoresis system to determine baculovirus titer.

The Trofile assay is used to determine HIV tropism.

One may use a viral plaque assay to calculate the number of viruses present in a sample. This technique requires counting the number of plaques formed by a virus sample, from which the actual virus concentration can be determined.

Cellular secretions

A wide range of cellular secretions (say, a specific antibody or cytokine) can be detected using the ELISA technique. The number of cells which secrete those particular substances can be determined using a related technique, the ELISPOT assay.

Drugs

See also

References

  1. ^ The American Heritage Dictionary of the English Language. Fourth Edition. Houghton Mifflin Company: 2000.
  2. ^ McKean, Erin (ed.). The New Oxford American Dictionary. Second edition. Oxford University Press: 2005.
  3. ^ Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (November 1951). "Protein measurement with the Folin phenol reagent". J. Biol. Chem. 193 (1): 265–75. PMID 14907713. http://www.jbc.org/cgi/pmidlookup?view=long&pmid=14907713.  
  4. ^ http://www.animal.ufl.edu/hansen/protocols/LOWRY.htm

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