CD20: Wikis


Note: Many of our articles have direct quotes from sources you can cite, within the Wikipedia article! This article doesn't yet, but we're working on it! See more info or our list of citable articles.


From Wikipedia, the free encyclopedia

Membrane-spanning 4-domains, subfamily A, member 1
Symbols MS4A1; B1; Bp35; CD20; LEU-16; MGC3969; MS4A2; S7
External IDs OMIM112210 MGI88321 HomoloGene7259 GeneCards: MS4A1 Gene
RNA expression pattern
PBB GE MS4A1 210356 x at tn.png
PBB GE MS4A1 217418 x at tn.png
More reference expression data
Species Human Mouse
Entrez 931 12482
Ensembl ENSG00000156738 ENSMUSG00000024673
UniProt P11836 Q542S5
RefSeq (mRNA) NM_021950 NM_007641
RefSeq (protein) NP_068769 NP_031667
Location (UCSC) Chr 11:
59.98 - 59.99 Mb
Chr 19:
11.32 - 11.33 Mb
PubMed search [2] [3]

CD20 is a non-glycosylated phosphoprotein expressed on the surface of all mature B-cells.

B-lymphocyte antigen CD20 is a protein that in humans is encoded by the MS4A1 gene.[1][2]

This gene encodes a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.[2]



The protein has no known natural ligand[3] and its function is unclear. It is suspected that it acts as a calcium channel in the cell membrane.


CD20 is expressed on all stages of B cell development except the first and last; it is present from pre-pre B cells through memory cells, but not on either pro-B cells or plasma cells.[4]

It is found on B-cell lymphomas, hairy cell leukemia, and B-cell chronic lymphocytic leukemia. It is also found on skin/melanoma cancer stem cells.[5]

Clinical significance

It is the target of the monoclonal antibodies rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias.

Additional anti-CD20 antibody therapeutics under development (phase II or III clinical trials) include AME-133v (Applied Molecular Evolution), Ocrelizumab (Roche) especially for rheumatoid arthritis, Ofatumumab (Genmab), TRU-015 (Trubion) and IMMU-106 (Immunomedics).[6]

FMC7 appears to be a conformational variant.[7]


  1. ^ Tedder TF, Streuli M, Schlossman SF, Saito H (Mar 1988). "Isolation and structure of a cDNA encoding the B1 (CD20) cell-surface antigen of human B lymphocytes". Proc Natl Acad Sci U S A 85 (1): 208-12. PMID 2448768.  
  2. ^ a b "Entrez Gene: MS4A1 membrane-spanning 4-domains, subfamily A, member 1".  
  3. ^ The biology of CD20 and its potential as a target for mAb therapy.[1]
  4. ^ Bona, Constantin; Francisco A. Bonilla (1996). "5". Textbook of Immunology. Martin Soohoo (2 ed.). CRC Press. p. 102. ISBN 9783718605965.  
  5. ^ Fang D, Nguyen TK, Leishear K, et al. (2005). "A tumorigenic subpopulation with stem cell properties in melanomas". Cancer Res. 65 (20): 9328–37. doi:10.1158/0008-5472.CAN-05-1343. PMID 16230395.  
  6. ^ Note: information included in this article only found in table present in print version of article. K. John Morrow Jr (2008-06-15). "Methods for Maximizing Antibody Yields". Genetic Engineering & Biotechnology News (Mary Ann Liebert, Inc.): p. 36. Retrieved 2008-07-06.  
  7. ^ Polyak MJ, Ayer LM, Szczepek AJ, Deans JP (2003). "A cholesterol-dependent CD20 epitope detected by the FMC7 antibody". Leukemia 17 (7): 1384–9. doi:10.1038/sj.leu.2402978. PMID 12835728.  

Further reading

  • Macardle PJ, Nicholson IC (2003). "CD20.". J. Biol. Regul. Homeost. Agents 16 (2): 136–8. PMID 12144126.  
  • Tamayose K, Sato N, Ando J, et al. (2002). "CD3-negative, CD20-positive T-cell prolymphocytic leukemia: case report and review of the literature.". Am. J. Hematol. 71 (4): 331–5. doi:10.1002/ajh.10224. PMID 12447967.  
  • Küster H, Zhang L, Brini AT, et al. (1992). "The gene and cDNA for the human high affinity immunoglobulin E receptor beta chain and expression of the complete human receptor.". J. Biol. Chem. 267 (18): 12782–7. PMID 1535625.  
  • Einfeld DA, Brown JP, Valentine MA, et al. (1988). "Molecular cloning of the human B cell CD20 receptor predicts a hydrophobic protein with multiple transmembrane domains.". EMBO J. 7 (3): 711–7. PMID 2456210.  
  • Tedder TF, Disteche CM, Louie E, et al. (1989). "The gene that encodes the human CD20 (B1) differentiation antigen is located on chromosome 11 near the t(11;14)(q13;q32) translocation site.". J. Immunol. 142 (7): 2555–9. PMID 2466898.  
  • Tedder TF, Klejman G, Schlossman SF, Saito H (1989). "Structure of the gene encoding the human B lymphocyte differentiation antigen CD20 (B1).". J. Immunol. 142 (7): 2560–8. PMID 2466899.  
  • Loken MR, Shah VO, Dattilio KL, Civin CI (1987). "Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development.". Blood 70 (5): 1316–24. PMID 3117132.  
  • Stamenkovic I, Seed B (1988). "Analysis of two cDNA clones encoding the B lymphocyte antigen CD20 (B1, Bp35), a type III integral membrane protein.". J. Exp. Med. 167 (6): 1975–80. doi:10.1084/jem.167.6.1975. PMID 3260267.  
  • Bofill M, Janossy G, Janossa M, et al. (1985). "Human B cell development. II. Subpopulations in the human fetus.". J. Immunol. 134 (3): 1531–8. PMID 3871452.  
  • Deans JP, Kalt L, Ledbetter JA, et al. (1995). "Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20.". J. Biol. Chem. 270 (38): 22632–8. PMID 7545683.  
  • Valentine MA, Licciardi KA, Clark EA, et al. (1993). "Insulin regulates serine/threonine phosphorylation in activated human B lymphocytes.". J. Immunol. 150 (1): 96–105. PMID 7678037.  
  • Bubien JK, Zhou LJ, Bell PD, et al. (1993). "Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes.". J. Cell Biol. 121 (5): 1121–32. doi:10.1083/jcb.121.5.1121. PMID 7684739.  
  • Shirakawa T, Li A, Dubowitz M, et al. (1994). "Association between atopy and variants of the beta subunit of the high-affinity immunoglobulin E receptor.". Nat. Genet. 7 (2): 125–9. doi:10.1038/ng0694-125. PMID 7920628.  
  • Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.  
  • Szepetowski P, Perucca-Lostanlen D, Gaudray P (1993). "Mapping genes according to their amplification status in tumor cells: contribution to the map of 11q13.". Genomics 16 (3): 745–50. doi:10.1006/geno.1993.1257. PMID 8325649.  
  • Algino KM, Thomason RW, King DE, et al. (1996). "CD20 (pan-B cell antigen) expression on bone marrow-derived T cells.". Am. J. Clin. Pathol. 106 (1): 78–81. PMID 8701937.  
  • Szöllósi J, Horejsí V, Bene L, et al. (1996). "Supramolecular complexes of MHC class I, MHC class II, CD20, and tetraspan molecules (CD53, CD81, and CD82) at the surface of a B cell line JY.". J. Immunol. 157 (7): 2939–46. PMID 8816400.  
  • Kanzaki M, Lindorfer MA, Garrison JC, Kojima I (1997). "Activation of the calcium-permeable cation channel CD20 by alpha subunits of the Gi protein.". J. Biol. Chem. 272 (23): 14733–9. doi:10.1074/jbc.272.23.14733. PMID 9169438.  
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.  

External links

Got something to say? Make a comment.
Your name
Your email address