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Portuguese royalty
House of Aviz-Beja
Flag Manuel I of Portugal.svg

Manuel I
Children include
   Miguel da Paz, Prince of Portugal and of Asturias
   John III
   Isabella, Holy Roman Empress
   Beatrice, Duchess of Savoy
   Louis, Duke of Beja
   Ferdinand, Duke of Guarda and Trancoso
   Cardinal-Infante Afonso
   Henry
   Edward, Duke of Guimarães
   Maria, Lady of Viseu
Grandchildren include
   Anthony (illegitimate)
   Maria, Duchess of Parma and Piacenza
   Catherine, Duchess of Braganza
Great-Great-Grandchildren include
   John IV
John III
Children include
   Maria Manuela, Princess of Portugal and of Asturias
   John Manuel, Prince of Portugal
Grandchildren include
   Sebastian
Sebastian
Henry
Anthony

The Cardinal-Infante Afonso (1509–1540; Portuguese pronunciation: [ɐˈfõsu]; English: Alphonzo) was a Portuguese infante (prince), son of King Manuel I of Portugal and his wife Maria of Aragon.

Afonso was born in Évora on April 23, 1509 and during his life was made Cardinal of the Kingdom after occupying several religious positions throughout the country.

He died on April 21, 1540, in Lisbon and was buried in Lisbon Cathedral before he was moved to the Jerónimos Monastery.

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