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Epitope mapping is the process of identification and characterization of the minimum molecular structures that are able to be recognized by the Immune System elements, mainly T and B cells. A collection of in vivo and in vitro methodologies are used for epitope mapping. Among the most used are binding assay, ELISPOT, HLA transgenic mice and prediction software. Epitope mapping of proteins is used for development of new vaccines and diagnostic approaches.

Epitope mapping is the process of matching surface proteins to antibodies that will bond to them.

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