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Evans blue
Evans blue.png
IUPAC name
CAS number 314-13-6
PubChem 6321418
MeSH Evans+blue
Molecular formula C34H24N6Na4O14S 4
Molar mass 960.81 g mol−1
Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

T-1824 or Evans Blue, also rendered as Evan's Blue, is an Azo Compound dye which has a very high affinity for serum albumin.

Because of this, it can be useful in physiology in estimating the proportion of body water contained in blood plasma.[1]

Evans blue is also used to assess the permeability of the blood-brain barrier to macromolecules. Because serum albumin cannot cross the barrier, and virtually all Evans Blue is bound to albumin, normally the neural tissue remains unstained.[2] When the BBB has been compromised, albumin-bound Evans blue enters the CNS. It fluoresces with excitation peaks at 470 and 540 nm and an emission peak at 680 nm. [3]

It was named after Herbert McLean Evans, an American chemist.

See also


  1. ^ Physiology at MCG 7/7ch02/7ch02p17
  2. ^ Hawkins BT, Egleton RD (2006). "Fluorescence imaging of blood-brain barrier disruption". Journal of Neuroscience Methods 151 (2): 262–7. doi:10.1016/j.jneumeth.2005.08.006.  
  3. ^ Hed J, Dahlgren C, and Rundquist, I (1983). "A Simple Fluorescence Technique to Stain the Plasma Membrane of Human Neutrophils". Histochemistry 79 (1): 105–10. doi:10.1007/BF00494347.  

External links

  • el-Sayed H, Goodall S, Hainsworth R (1995). "Re-evaluation of Evans blue dye dilution method of plasma volume measurement". Clin Lab Haematol 17 (2): 189–94. PMID 8536425.  


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