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ITS (for internal transcribed spacer) refers to a piece of non-functional RNA situated between structural ribosomal RNAs (rRNA) on a common precursor transcript. Read from 5' to 3', this polycistronic rRNA precursor transcript contains the 5' external transcribed sequence (5' ETS), 18S rRNA, ITS1, 5.8S rRNA, ITS2, 26S rRNA and finally the 3'ETS. During rRNA maturation, ETS and ITS pieces are excised and as non-functional maturation by-products rapidly degraded. Genes encoding ribosomal rRNA and spacers occur in tandem repeats that are thousands of copies long, each separated by regions of non-transcribed DNA termed intergenic spacer (IGS) or non-transcribed spacer (NTS). Sequence comparison of the ITS region is widely used in taxonomy and molecular phylogeny because it a) is (due to the high copy number of rRNA genes) easy to amplify even from small quantities of DNA, and b) has a high degree of variation even between closely related species. This can be explained by the relatively low evolutionary pressure acting on such non-functional sequences.

For example, ITS has proven especially useful for elucidating relationships among congeneric species and closely related genera in Asteraceae [1](Baldwin, 1992; Baldwin et al., 1995; Kim et al., 1996) as well as clinically important yeast species.[2]

The ITS region is now perhaps the most widely sequenced DNA region in fungi. It has typically been most useful for molecular systematics at the species level, and even within species (e.g., to identify geographic races). Because of its higher degree of variation than other genic regions of rDNA (for small- and large-subunit rRNA), variation among individual rDNA repeats can sometimes be observed within both the ITS and IGS regions. In addition to the standard ITS1+ITS4 primers used by most labs, several taxon-specific primers have been described that allow selective amplification of fungal sequences (e.g., see Gardes & Bruns 1993 paper describing amplification of basidiomycete ITS sequences from mycorrhiza samples)[3]. ITS region is nowadays being used to know the genetic diversity among different strains of bacteria by sequencing the ITS gene.

References

  1. ^ Baldwin, B.G. (1992). "Phylogenetic utility of the internal transcribed spacers of nuclear ribosomal DNA in plants: An example from the Compositaogy". Molecular Phylogenetics and Evolution 1: 3–16. doi:10.1016/1055-7903(92)90030-K.  
  2. ^ Chen,Y-C, J. D. Eisner, M. M. Kattar, S. L. Rassoulian-Barrett, K. Lafe, A. P. Limaye, and B. T. Cookson (2001). "Polymorphic Internal Transcribed Spacer Region 1 DNA Sequences Identify Medically Important Yeasts". J. Clin. Microbiol. 39: 4042–4051. doi:10.1128/JCM.39.11.4042-4051.2001. PMID 11682528.  
  3. ^ Gardes, M., and Bruns, T.D. (1993). "ITS primers with enhanced specificity for basidiomycetes: application to the identification of mycorrhiza and rusts". Molecular Ecology. 2: 113–118. doi:10.1111/j.1365-294X.1993.tb00005.x. PMID 8180733.  

External links

University of Washington Laboratory Medicine: Molecular Diagnosis | Yeast Sequencing

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