Born in Rouyn, Quebec, Bissonnette studied education science at the Université de Montréal from 1965 to 1970. She later pursed doctoral studies at the University of Strasbourg and the École pratique des hautes études in Paris. In 1974, she became a reporter for the daily newspaper Le Devoir. She became the parliamentary correspondent in Quebec City, then in Ottawa, before taking on the position of editorialist and, finally, that of writer-in-chief in 1982. From 1986 to 1990, she worked as an independent journalist and consultant, and collaborated with many Quebec and Canadian media organizations. She writes a weekly article on Quebec affairs for the Canadian daily newspaper The Globe and Mail, as well as monthly articles for the magazines L'actualité and Montreal Magazine. In 1990, she returned to Le Devoir, where she served as editor-in-chief until 1998. Bissonnette appears regularly as an analyst on both French and English news programs on public and private radio and television networks.
Bissonnette received the Order of Francophones of America in 1993. She was elected member of the Academy of Letters and the Social sciences of the Royal Society of Canada in 1994. In 1998 she was made an Officer of the National Order of Quebec. She holds five doctorates honoris causa: from the Université de Sherbrooke, State University of New York, Concordia University, Laurentian University and Université Laval and the Université de Montréal. In 2000, she was awarded the French Légion d'honneur, and received the new mérite d'honneur of French and of Francophone education, bestowed by the Quebec Minister of International Relations, Louise Beaudoin. She is a member of the Union of Quebec Writers.
Lise Bissonnette is the president and general director of the Bibliothèque et Archives nationales du Québec, formerly known as the Bibliothèque nationale du Québec. She was appointed to this position in 1998. She leaves her post after 11 years of service.