The Full Wiki

Microtubule: Wikis

Advertisements
  

Note: Many of our articles have direct quotes from sources you can cite, within the Wikipedia article! This article doesn't yet, but we're working on it! See more info or our list of citable articles.

Encyclopedia

From Wikipedia, the free encyclopedia

Space-filling model of a microtubule segment derived from cryo-electron microscopy. The protofilaments are seen running along the axis of the segment. The microtubule (+) end is towards the top of the image.[1]

Microtubules are one of the components of the cytoskeleton. They have a diameter of 25 nm and length varying from 200 nanometers to 25 micrometers. Microtubules serve as structural components within cells and are involved in many cellular processes including mitosis, cytokinesis, and vesicular transport.[2]

Contents

Structure

Microtubules are polymers of α- and β-tubulin dimers. The tubulin dimers polymerize end to end in protofilaments. The protofilaments then bundle into hollow cylindrical filaments. Typically, the protofilaments arrange themselves in an imperfect helix with one turn of the helix containing 13 tubulin dimers each from a different protofilament. The image above illustrates a small section of microtubule, a few αβ dimers in length.

Another important feature of microtubule structure is polarity. Tubulin polymerizes end to end with the α subunit of one tubulin dimer contacting the β subunit of the next. Therefore, in a protofilament, one end will have the α subunit exposed while the other end will have the β subunit exposed. These ends are designated the (−) and (+) ends, respectively. The protofilaments bundle parallel to one another, so in a microtubule, there is one end, the (+) end, with only β subunits exposed while the other end, the (−) end, only has α subunits exposed.

Organization within cells

Microtubules are nucleated and organized by the microtubule organizing centers (MTOCs), such as centrioles and basal bodies. Microtubules are part of a structural network (the cytoskeleton) within the cell's cytoplasm, but, in addition to structural support, microtubules take part in many other processes, as well. They are capable of growing and shrinking in order to generate force, and there are also motor proteins that allow organelles and other cellular factors to move along the microtubule. A notable structure involving microtubules is the mitotic spindle used by eukaryotic cells to segregate their chromosomes correctly during cell division. Microtubules are also part of the cilia and flagella of eukaryotic cells (prokaryote flagella are entirely different).

Nucleation and growth

Polymerization of microtubules is nucleated in a microtubule organizing center. Contained within the MTOC is another type of tubulin, γ-tubulin, which is distinct from the α and β subunits which compose the microtubules themselves. The γ-tubulin combines with several other associated proteins to form a circular structure known as the "γ-tubulin ring complex." This complex acts as a scaffold for α/β tubulin dimers to begin polymerization; it acts as a cap of the (−) end while microtubule growth continues away from the MTOC in the (+) direction.

Dynamic instability

During polymerization, both the α- and β-subunits of the tubulin dimer are bound to a molecule of GTP. While the GTP bound to α-tubulin is stable, the GTP bound to β-tubulin may be hydrolyzed to GDP shortly after assembly. The kinetics of GDP-tubulin are different from those of GTP-tubulin; GDP-tubulin is prone to depolymerization. A GDP-bound tubulin subunit at the tip of a microtubule will fall off, though a GDP-bound tubulin in the middle of a microtubule cannot spontaneously pop out. Since tubulin adds onto the end of the microtubule only in the GTP-bound state, there is generally a cap of GTP-bound tubulin at the tip of the microtubule, protecting it from disassembly. When hydrolysis catches up to the tip of the microtubule, it begins a rapid depolymerization and shrinkage. This switch from growth to shrinking is called a catastrophe. GTP-bound tubulin can begin adding to the tip of the microtubule again, providing a new cap and protecting the microtubule from shrinking. This is referred to as rescue.[3]

Dynamic Instability

In vivo microtubule dynamics vary considerably. Assembly, disassembly and catastrophe rates depend on which microtubule-associated proteins (MAPs) are present.

Advertisements

Chemical effects on microtubule dynamics

Microtubule dynamics can also be altered by drugs.

  • For example, the taxane drug class (e.g. paclitaxel or docetaxel), used in the treatment of cancer, blocks dynamic instability by stabilizing GDP-bound tubulin in the microtubule. Thus, even when hydrolysis of GTP reaches the tip of the microtubule, there is no depolymerization and the microtubule does not shrink back.
  • Nocodazole and Colchicine have the opposite effect, blocking the polymerization of tubulin into microtubules.

Motor proteins

In addition to movement generated by the dynamic instability of the microtubule itself, the fibers are substrates along which motor proteins can move. The major microtubule motor proteins are kinesin, which generally moves towards the (+) end of the microtubule, and dynein, which generally moves towards the (−) end.

Additional images

References

  • Manu Lopus, Mythili Yenjerla, and Leslie Wilson (2009) Microtubule Dynamics (Advanced Review). In Wiley Encyclopedia of Chemical Biology, Begley TP, Ed. Wiley, NJ. 3, 153–160.
  1. ^ Li H, DeRosier DJ, Nicholson WV, Nogales E, Downing KH (October 2002). "Microtubule structure at 8 Å resolution". Structure 10 (10): 1317–28. doi:10.1016/S0969-2126(02)00827-4. PMID 12377118. 
  2. ^ "Microtubule - Definitions from Dictionary.com". dictionary.reference.com. http://dictionary.reference.com/browse/Microtubule. Retrieved 2008-05-15. 
  3. ^ Mitchison T, Kirschner M (15-21 November 1984). "Dynamic instability of microtubule growth". Nature 312 (5991): 237–42. PMID 6504138. 

Advertisements






Got something to say? Make a comment.
Your name
Your email address
Message