|Maintained by MoDOT|
|Length:||110 mi (177 km)|
|North end:||U.S. Route 54/Route DD in Cedar Springs|
|South end:||Highway 221 at the Arkansas state line|
|Major cities:||Cedar Springs
Route 39 is a two-lane highway in southwest Missouri. Its northern terminus is at U.S. Route 54 between Collins and El Dorado Springs at the community of Cedar Springs; its southern terminus is at the Arkansas state line (north of Berryville, Arkansas) where it continues as Highway 221.
There is a short Business Route 39 in Mount Vernon.
Route 39 begins at US 54, heading south-southeast towards Stockton. About one mile (1.6 km) before reaching Stockton, the road begins a brief concurrency with Route 32 before turning south again. Two miles south of Stockton is the western terminus of Route 215. South of Arcola, Route 39 crosses a branch of Stockton Lake.
At Greenfield is the beginning of a concurrency with U.S. Route 160 which last for two miles (3 km), with US 160 turning west and Route 39 continuing south to South Greenfield. At Albatross is an intersection with Route 96. The curves around on its approach to Mount Vernon.
At the north edge of Mount Vernon is the western terminus of Route 174, and two miles (3 km) further south, Route 39 turns east, joining Business Loop I-44 for about two miles (3 km). At Interstate 44, the business loop ends, and Route 265 begins and the two will be united to Aurora, where Route 39 intersects U.S. Route 60.
South of Aurora, Route 39 heads straight south for about sixteen miles and intersects Route 248. The highway then enters the Mark Twain National Forest. Eight miles south of Route 248, Route 39 forms a brief concurrency with Route 76, then turns south again. At Shell Knob, Route 39 crosses Table Rock Lake. At Carr Lane is an intersection with Route 86, and one mile (1.6 km) further south the highway crosses the Arkansas state line.