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The Orto Botanico "Pierina Scaramella" (2,200 m²), also known as the Orto Botanico dell'Università di Urbino, is a botanical garden maintained by the University of Urbino, and located at via Bramante, 28, Urbino, Marche, Italy. Entrance is free.

The garden was established in 1806 by Giovanni De Brignole. It was organized into two major sections with trees at the higher elevation and rectangular flower beds below. A large greenhouse was built in 1811, and in 1812 the garden's first catalog listed 2731 species.

Today the garden contains medicinal and ornamental plants such as Ailanthus glandulosus, Sophora japonica, and Cycadaceae and Gymnospermae. Trees include Acer pseudo−platanus, Fagus silvatica, Fraxinus excelsior, Liriodendron tulipifera, Quercus ilex, and Taxus baccata.

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