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P-selectin glycoprotein ligand-1: Wikis


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Selectin P ligand
Symbols SELPLG; CD162; CLA; PSGL-1; PSGL1
External IDs OMIM600738 MGI106689 HomoloGene2261 GeneCards: SELPLG Gene
RNA expression pattern
PBB GE SELPLG 209879 at tn.png
PBB GE SELPLG 209880 s at tn.png
More reference expression data
Species Human Mouse
Entrez 6404 20345
Ensembl ENSG00000110876 ENSMUSG00000048163
UniProt Q14242 Q99L34
RefSeq (mRNA) NM_003006 NM_009151
RefSeq (protein) NP_002997 NP_033177
Location (UCSC) Chr 12:
107.54 - 107.54 Mb
Chr 5:
114.08 - 114.09 Mb
PubMed search [1] [2]

Selectin P ligand, also known as SELPLG or CD162 (cluster of differentiation 162), is a human gene.

SELPLG is the high affinity counter-receptor for P-selectin on myeloid cells and stimulated T lymphocytes. As such, it plays a critical role in the tethering of these cells to activated platelets or endothelia expressing P-selectin. The organization of the SELPG gene closely resembles that of CD43 and the human platelet glycoprotein GpIb-alpha both of which have an intron in the 5-prime-noncoding region, a long second exon containing the complete coding region, and TATA-less promoters.[1]

P-selectin glycoprotein ligand-1 is a glycoprotein found on white blood cells and endothelial cells that binds to P-selectin (P stands for platelet). It is one of a family of selectins that includes E-selectin (endothelial) and L-selectin (leukocyte). Selectins are part of the broader family of cell adhesion molecules. PSGL-1 can bind to all three members of the family but binds best (with the highest affinity) to P-selectin.


Posttranslational modification

PSGL-1 protein requires two distinct posttranslational modifications to gain its selectin binding activity:


PSGL-1 is expressed on all white blood cells and plays an important role in the recruitment of white blood cells into inflamed tissue.

White blood cells normally do not interact with the endothelium of blood vessels. However, inflammation causes the expression of cell adhesion molecules (CAM) such as P-selectin on the surface of the blood vessel wall. White blood cells present in flowing blood can interact with CAM. The first step in this interaction process is carried out by PSGL-1 interacting with P-selectin and/or E-selectin on endothelial cells and adherent platelets. This interaction results in "rolling" of the white blood cell on the endothelial cell surface followed by stable adhesion and transmigration of the white blood cell into the inflamed tissue.


Further reading

{{PBB_Further_reading | citations =

  • Furie B, Furie BC (2004). "Role of platelet P-selectin and microparticle PSGL-1 in thrombus formation.". Trends in molecular medicine 10 (4): 171–8. doi:10.1016/j.molmed.2004.02.008. PMID 15059608.  
  • Sako D, Chang XJ, Barone KM, et al. (1993). "Expression cloning of a functional glycoprotein ligand for P-selectin.". Cell 75 (6): 1179–86. doi:10.1016/0092-8674(93)90327-M. PMID 7505206.  
  • Moore KL, Eaton SF, Lyons DE, et al. (1994). "The P-selectin glycoprotein ligand from human neutrophils displays sialylated, fucosylated, O-linked poly-N-acetyllactosamine.". J. Biol. Chem. 269 (37): 23318–27. PMID 7521878.  
  • Veldman GM, Bean KM, Cumming DA, et al. (1995). "Genomic organization and chromosomal localization of the gene encoding human P-selectin glycoprotein ligand.". J. Biol. Chem. 270 (27): 16470–5. doi:10.1074/jbc.270.27.16470. PMID 7541799.  
  • Wilkins PP, Moore KL, McEver RP, Cummings RD (1995). "Tyrosine sulfation of P-selectin glycoprotein ligand-1 is required for high affinity binding to P-selectin.". J. Biol. Chem. 270 (39): 22677–80. doi:10.1074/jbc.270.39.22677. PMID 7559387.  
  • Sako D, Comess KM, Barone KM, et al. (1995). "A sulfated peptide segment at the amino terminus of PSGL-1 is critical for P-selectin binding.". Cell 83 (2): 323–31. doi:10.1016/0092-8674(95)90173-6. PMID 7585949.  
  • Pouyani T, Seed B (1995). "PSGL-1 recognition of P-selectin is controlled by a tyrosine sulfation consensus at the PSGL-1 amino terminus.". Cell 83 (2): 333–43. doi:10.1016/0092-8674(95)90174-4. PMID 7585950.  
  • Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides.". Gene 138 (1-2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.  
  • Li F, Wilkins PP, Crawley S, et al. (1996). "Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin.". J. Biol. Chem. 271 (6): 3255–64. doi:10.1074/jbc.271.6.3255. PMID 8621728.  
  • Guyer DA, Moore KL, Lynam EB, et al. (1996). "P-selectin glycoprotein ligand-1 (PSGL-1) is a ligand for L-selectin in neutrophil aggregation.". Blood 88 (7): 2415–21. PMID 8839831.  
  • Goetz DJ, Greif DM, Ding H, et al. (1997). "Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin.". J. Cell Biol. 137 (2): 509–19. doi:10.1083/jcb.137.2.509. PMID 9128259.  
  • Fuhlbrigge RC, Kieffer JD, Armerding D, Kupper TS (1997). "Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells.". Nature 389 (6654): 978–81. doi:10.1038/40166. PMID 9353122.  
  • Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library.". Gene 200 (1-2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.  
  • Snapp KR, Ding H, Atkins K, et al. (1998). "A novel P-selectin glycoprotein ligand-1 monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL-1 and blocks recognition of both P- and L-selectin.". Blood 91 (1): 154–64. PMID 9414280.  
  • Bennett EP, Hassan H, Mandel U, et al. (1998). "Cloning of a human UDP-N-acetyl-alpha-D-Galactosamine:polypeptide N-acetylgalactosaminyltransferase that complements other GalNAc-transferases in complete O-glycosylation of the MUC1 tandem repeat.". J. Biol. Chem. 273 (46): 30472–81. doi:10.1074/jbc.273.46.30472. PMID 9804815.  
  • Wimazal F, Ghannadan M, Müller MR, et al. (2000). "Expression of homing receptors and related molecules on human mast cells and basophils: a comparative analysis using multi-color flow cytometry and toluidine blue/immunofluorescence staining techniques.". Tissue Antigens 54 (5): 499–507. doi:10.1034/j.1399-0039.1999.540507.x. PMID 10599889.  
  • Epperson TK, Patel KD, McEver RP, Cummings RD (2000). "Noncovalent association of P-selectin glycoprotein ligand-1 and minimal determinants for binding to P-selectin.". J. Biol. Chem. 275 (11): 7839–53. doi:10.1074/jbc.275.11.7839. PMID 10713099.  
  • André P, Spertini O, Guia S, et al. (2000). "Modification of P-selectin glycoprotein ligand-1 with a natural killer cell-restricted sulfated lactosamine creates an alternate ligand for L-selectin.". Proc. Natl. Acad. Sci. U.S.A. 97 (7): 3400–5. doi:10.1073/pnas.040569797. PMID 10725346.  
  • Frenette PS, Denis CV, Weiss L, et al. (2000). "P-Selectin glycoprotein ligand 1 (PSGL-1) is expressed on platelets and can mediate platelet-endothelial interactions in vivo.". J. Exp. Med. 191 (8): 1413–22. doi:10.1084/jem.191.8.1413. PMID 10770806.  
  • Woltmann G, McNulty CA, Dewson G, et al. (2000). "Interleukin-13 induces PSGL-1/P-selectin-dependent adhesion of eosinophils, but not neutrophils, to human umbilical vein endothelial cells under flow.". Blood 95 (10): 3146–52. PMID 10807781.  
  • Tsuchihashi S, Fondevila C, Shaw GD, et al. (2006). "Molecular characterization of rat leukocyte P-selectin glycoprotein ligand-1 and effect of its blockade: protection from ischemia-reperfusion injury in liver transplantation.". J Immunol 176 (1): 616–24. PMID 16365457.  

External links

This article incorporates text from the United States National Library of Medicine, which is in the public domain.



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