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P300/CBP-associated factor

PDB rendering based on 1cm0.
Available structures
1cm0, 1jm4, 1n72, 1wug, 1wum, 1zs5
Identifiers
Symbols PCAF; KAT2B; P/CAF; GCN5; GCN5L
External IDs OMIM602303 MGI1343094 HomoloGene20834 GeneCards: PCAF Gene
RNA expression pattern
PBB GE PCAF 203845 at tn.png
More reference expression data
Orthologs
Species Human Mouse
Entrez 8850 18519
Ensembl ENSG00000114166 ENSMUSG00000000708
UniProt Q92831 n/a
RefSeq (mRNA) XM_001130666 XM_001003318
RefSeq (protein) XP_001130666 XP_001003318
Location (UCSC) Chr 3:
20.06 - 20.17 Mb
Chr 17:
53.03 - 53.14 Mb
PubMed search [1] [2]

P300/CBP-associated factor (PCAF), also known as K(lysine) acetyltransferase 2B (KAT2B), is a human gene and trancriptional coactivator associated with p53.

Contents

Structure

Several domains of PCAF can act independently or in unison to enable its functions. PCAF has separate acetyltransferase and E3 ubiquitin ligase domains as well as a bromodomain for interaction with other proteins. PCAF also possesses sites for its own acetylation and ubiquitination.[1]

Function

CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by the PCAF gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.[2]

Regulation

The acetyltransferase activity and cellular location of PCAF are regulated through acetylation of PCAF itself. PCAF may be autoacetylated (acetylated by itself) or by p300. Acetylation leads to migration to the nucleus and enhances its acetyltransferase activity.[3] PCAF interacts with and is deacetylated by HDAC3, leading to a reduction in PCAF acetyltransferase activity and cytoplasmic localisation.[4]

Protein interactions

PCAF forms complexes with numerous proteins which guide its activity. For example PCAF is recruited by ATF[5] to acetylate histones and promote transcription of ATF4 target genes.

Targets

There are various protein targets of PCAF's acetyltransferase activity including transcription factors such as Fli1[6], p53[7] and numerous histone residues. Hdm2, itself a ubiquitin ligase which targets p53, has also been demonstrated to be a target of the ubiquitin-ligase activity of PCAF.[1]

Interactions

PCAF has been shown to interact with Beta-catenin,[8] Twist transcription factor,[9] Myc,[10] IRF1,[11] IRF2,[12][11] Transformation/transcription domain-associated protein,[10][13] CREB binding protein,[14][15] BRCA2,[16][17] KLF13,[18] TCF3,[19] RBPJ,[20] POLR2A,[15] Nuclear receptor coactivator 1,[21] EVI1,[22] Mdm2[23] and HNF1A.[24]

See also

References

  1. ^ a b Linares LK, Kiernan R, Triboulet R, et al. (March 2007). "Intrinsic ubiquitination activity of PCAF controls the stability of the oncoprotein Hdm2". Nat. Cell Biol. 9 (3): 331–8. doi:10.1038/ncb1545. PMID 17293853.  
  2. ^ "Entrez Gene: PCAF p300/CBP-associated factor". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8850.  
  3. ^ Santos-Rosa H, Valls E, Kouzarides T, Martínez-Balbás M (August 2003). "Mechanisms of P/CAF auto-acetylation". Nucleic Acids Res. 31 (15): 4285–92. doi:10.1093/nar/gkg655. PMID 12888487. PMC 169960. http://nar.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=12888487.  
  4. ^ Grégoire S, Xiao L, Nie J, et al. (February 2007). "Histone deacetylase 3 interacts with and deacetylates myocyte enhancer factor 2". Mol. Cell. Biol. 27 (4): 1280–95. doi:10.1128/MCB.00882-06. PMID 17158926.  
  5. ^ Chérasse Y, Maurin AC, Chaveroux C, et al. (2007). "The p300/CBP-associated factor (PCAF) is a cofactor of ATF4 for amino acid-regulated transcription of CHOP". Nucleic Acids Res. 35 (17): 5954–65. doi:10.1093/nar/gkm642. PMID 17726049. PMC 2034469. http://nar.oxfordjournals.org/cgi/pmidlookup?view=long&pmid=17726049.  
  6. ^ Asano Y, Czuwara J, Trojanowska M (November 2007). "Transforming growth factor-beta regulates DNA binding activity of transcription factor Fli1 by p300/CREB-binding protein-associated factor-dependent acetylation". J. Biol. Chem. 282 (48): 34672–83. doi:10.1074/jbc.M703907200. PMID 17884818.  
  7. ^ Liu L, Scolnick DM, Trievel RC, et al. (February 1999). "p53 sites acetylated in vitro by PCAF and p300 are acetylated in vivo in response to DNA damage". Mol. Cell. Biol. 19 (2): 1202–9. PMID 9891054. PMC 116049. http://mcb.asm.org/cgi/pmidlookup?view=long&pmid=9891054.  
  8. ^ Ge, Xinjian; Jin Qihuang, Zhang Fang, Yan Tingting, Zhai Qiwei (Jan. 2009). "PCAF acetylates {beta}-catenin and improves its stability". Mol. Biol. Cell (United States) 20 (1): 419–27. doi:10.1091/mbc.E08-08-0792. PMID 18987336.  
  9. ^ Hamamori, Y; Sartorelli V, Ogryzko V, Puri P L, Wu H Y, Wang J Y, Nakatani Y, Kedes L (Feb. 1999). "Regulation of histone acetyltransferases p300 and PCAF by the bHLH protein twist and adenoviral oncoprotein E1A". Cell (UNITED STATES) 96 (3): 405–13. ISSN 0092-8674. PMID 10025406.  
  10. ^ a b Liu, Xiaohui; Tesfai Jerusalem, Evrard Yvonne A, Dent Sharon Y R, Martinez Ernest (May. 2003). "c-Myc transformation domain recruits the human STAGA complex and requires TRRAP and GCN5 acetylase activity for transcription activation". J. Biol. Chem. (United States) 278 (22): 20405–12. doi:10.1074/jbc.M211795200. ISSN 0021-9258. PMID 12660246.  
  11. ^ a b Masumi, A; Wang I M, Lefebvre B, Yang X J, Nakatani Y, Ozato K (Mar. 1999). "The histone acetylase PCAF is a phorbol-ester-inducible coactivator of the IRF family that confers enhanced interferon responsiveness". Mol. Cell. Biol. (UNITED STATES) 19 (3): 1810–20. ISSN 0270-7306. PMID 10022868.  
  12. ^ Masumi, A; Ozato K (Jun. 2001). "Coactivator p300 acetylates the interferon regulatory factor-2 in U937 cells following phorbol ester treatment". J. Biol. Chem. (United States) 276 (24): 20973–80. doi:10.1074/jbc.M101707200. ISSN 0021-9258. PMID 11304541.  
  13. ^ Fuchs, M; Gerber J, Drapkin R, Sif S, Ikura T, Ogryzko V, Lane W S, Nakatani Y, Livingston D M (Aug. 2001). "The p400 complex is an essential E1A transformation target". Cell (United States) 106 (3): 297–307. ISSN 0092-8674. PMID 11509179.  
  14. ^ Tini, Marc; Benecke Arndt, Um Soo-Joong, Torchia Joseph, Evans Ronald M, Chambon Pierre (Feb. 2002). "Association of CBP/p300 acetylase and thymine DNA glycosylase links DNA repair and transcription". Mol. Cell (United States) 9 (2): 265–77. ISSN 1097-2765. PMID 11864601.  
  15. ^ a b Cho, H; Orphanides G, Sun X, Yang X J, Ogryzko V, Lees E, Nakatani Y, Reinberg D (Sep. 1998). "A human RNA polymerase II complex containing factors that modify chromatin structure". Mol. Cell. Biol. (UNITED STATES) 18 (9): 5355–63. ISSN 0270-7306. PMID 9710619.  
  16. ^ Lin, Horng-Ru; Ting Nicholas S Y, Qin Jun, Lee Wen-Hwa (Sep. 2003). "M phase-specific phosphorylation of BRCA2 by Polo-like kinase 1 correlates with the dissociation of the BRCA2-P/CAF complex". J. Biol. Chem. (United States) 278 (38): 35979–87. doi:10.1074/jbc.M210659200. ISSN 0021-9258. PMID 12815053.  
  17. ^ Fuks, F; Milner J, Kouzarides T (Nov. 1998). "BRCA2 associates with acetyltransferase activity when bound to P/CAF". Oncogene (ENGLAND) 17 (19): 2531–4. doi:10.1038/sj.onc.1202475. ISSN 0950-9232. PMID 9824164.  
  18. ^ Song, Chao-Zhong; Keller Kimberly, Murata Ken, Asano Haruhiko, Stamatoyannopoulos George (Mar. 2002). "Functional interaction between coactivators CBP/p300, PCAF, and transcription factor FKLF2". J. Biol. Chem. (United States) 277 (9): 7029–36. doi:10.1074/jbc.M108826200. ISSN 0021-9258. PMID 11748222.  
  19. ^ Bradney, Curtis; Hjelmeland Mark, Komatsu Yasuhiko, Yoshida Minoru, Yao Tso-Pang, Zhuang Yuan (Jan. 2003). "Regulation of E2A activities by histone acetyltransferases in B lymphocyte development". J. Biol. Chem. (United States) 278 (4): 2370–6. doi:10.1074/jbc.M211464200. ISSN 0021-9258. PMID 12435739.  
  20. ^ Kurooka, H; Honjo T (Jun. 2000). "Functional interaction between the mouse notch1 intracellular region and histone acetyltransferases PCAF and GCN5". J. Biol. Chem. (UNITED STATES) 275 (22): 17211–20. doi:10.1074/jbc.M000909200. ISSN 0021-9258. PMID 10747963.  
  21. ^ Spencer, T E; Jenster G, Burcin M M, Allis C D, Zhou J, Mizzen C A, McKenna N J, Onate S A, Tsai S Y, Tsai M J, O'Malley B W (Sep. 1997). "Steroid receptor coactivator-1 is a histone acetyltransferase". Nature (ENGLAND) 389 (6647): 194–8. doi:10.1038/38304. ISSN 0028-0836. PMID 9296499.  
  22. ^ Chakraborty, S; Senyuk V, Sitailo S, Chi Y, Nucifora G (Nov. 2001). "Interaction of EVI1 with cAMP-responsive element-binding protein-binding protein (CBP) and p300/CBP-associated factor (P/CAF) results in reversible acetylation of EVI1 and in co-localization in nuclear speckles". J. Biol. Chem. (United States) 276 (48): 44936–43. doi:10.1074/jbc.M106733200. ISSN 0021-9258. PMID 11568182.  
  23. ^ Jin, Yetao; Zeng Shelya X, Dai Mu-Shui, Yang Xiang-Jiao, Lu Hua (Aug. 2002). "MDM2 inhibits PCAF (p300/CREB-binding protein-associated factor)-mediated p53 acetylation". J. Biol. Chem. (United States) 277 (34): 30838–43. doi:10.1074/jbc.M204078200. ISSN 0021-9258. PMID 12068014.  
  24. ^ Soutoglou, E; Papafotiou G, Katrakili N, Talianidis I (Apr. 2000). "Transcriptional activation by hepatocyte nuclear factor-1 requires synergism between multiple coactivator proteins". J. Biol. Chem. (UNITED STATES) 275 (17): 12515–20. ISSN 0021-9258. PMID 10777539.  

External links

Further reading

  • Marcello A, Zoppé M, Giacca M (2002). "Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator.". IUBMB Life 51 (3): 175–81. doi:10.1080/152165401753544241. PMID 11547919.  
  • Ott M, Dorr A, Hetzer-Egger C, et al. (2004). "Tat acetylation: a regulatory switch between early and late phases in HIV transcription elongation.". Novartis Found. Symp. 259: 182–93; discussion 193–6, 223–5. doi:10.1002/0470862637.ch13. PMID 15171254.  
  • Liou LY, Herrmann CH, Rice AP (2005). "HIV-1 infection and regulation of Tat function in macrophages.". Int. J. Biochem. Cell Biol. 36 (9): 1767–75. doi:10.1016/j.biocel.2004.02.018. PMID 15183343.  
  • Gibellini D, Vitone F, Schiavone P, Re MC (2005). "HIV-1 tat protein and cell proliferation and survival: a brief review.". New Microbiol. 28 (2): 95–109. PMID 16035254.  
  • Hetzer C, Dormeyer W, Schnölzer M, Ott M (2006). "Decoding Tat: the biology of HIV Tat posttranslational modifications.". Microbes Infect. 7 (13): 1364–9. doi:10.1016/j.micinf.2005.06.003. PMID 16046164.  
  • Peruzzi F (2006). "The multiple functions of HIV-1 Tat: proliferation versus apoptosis.". Front. Biosci. 11: 708–17. doi:10.2741/1829. PMID 16146763.  
  • Stevens M, De Clercq E, Balzarini J (2007). "The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention.". Med Res Rev 26 (5): 595–625. doi:10.1002/med.20081. PMID 16838299.  
  • Harrich D, McMillan N, Munoz L, et al. (2007). "Will diverse Tat interactions lead to novel antiretroviral drug targets?". Current drug targets 7 (12): 1595–606. doi:10.2174/138945006779025338. PMID 17168834.  
  • Dawson SJ, White LA (1992). "Treatment of Haemophilus aphrophilus endocarditis with ciprofloxacin.". J. Infect. 24 (3): 317–20. doi:10.1016/S0163-4453(05)80037-4. PMID 1602151.  
  • Yang XJ, Ogryzko VV, Nishikawa J, et al. (1996). "A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A.". Nature 382 (6589): 319–24. doi:10.1038/382319a0. PMID 8684459.  
  • Ogryzko VV, Schiltz RL, Russanova V, et al. (1997). "The transcriptional coactivators p300 and CBP are histone acetyltransferases.". Cell 87 (5): 953–9. doi:10.1016/S0092-8674(00)82001-2. PMID 8945521.  
  • Jenster G, Spencer TE, Burcin MM, et al. (1997). "Steroid receptor induction of gene transcription: a two-step model.". Proc. Natl. Acad. Sci. U.S.A. 94 (15): 7879–84. doi:10.1073/pnas.94.15.7879. PMID 9223281.  
  • Chen H, Lin RJ, Schiltz RL, et al. (1997). "Nuclear receptor coactivator ACTR is a novel histone acetyltransferase and forms a multimeric activation complex with P/CAF and CBP/p300.". Cell 90 (3): 569–80. doi:10.1016/S0092-8674(00)80516-4. PMID 9267036.  
  • Spencer TE, Jenster G, Burcin MM, et al. (1997). "Steroid receptor coactivator-1 is a histone acetyltransferase.". Nature 389 (6647): 194–8. doi:10.1038/38304. PMID 9296499.  
  • Takeshita A, Cardona GR, Koibuchi N, et al. (1997). "TRAM-1, A novel 160-kDa thyroid hormone receptor activator molecule, exhibits distinct properties from steroid receptor coactivator-1.". J. Biol. Chem. 272 (44): 27629–34. doi:10.1074/jbc.272.44.27629. PMID 9346901.  
  • Korzus E, Torchia J, Rose DW, et al. (1998). "Transcription factor-specific requirements for coactivators and their acetyltransferase functions.". Science 279 (5351): 703–7. doi:10.1126/science.279.5351.703. PMID 9445475.  
  • Puri PL, Sartorelli V, Yang XJ, et al. (1998). "Differential roles of p300 and PCAF acetyltransferases in muscle differentiation.". Mol. Cell 1 (1): 35–45. doi:10.1016/S1097-2765(00)80005-2. PMID 9659901.  
  • Ogryzko VV, Kotani T, Zhang X, et al. (1998). "Histone-like TAFs within the PCAF histone acetylase complex.". Cell 94 (1): 35–44. doi:10.1016/S0092-8674(00)81219-2. PMID 9674425.  
  • Randhawa GS, Bell DW, Testa JR, Feinberg AP (1998). "Identification and mapping of human histone acetylation modifier gene homologues.". Genomics 51 (2): 262–9. doi:10.1006/geno.1998.5370. PMID 9722949.  
  • Benkirane M, Chun RF, Xiao H, et al. (1998). "Activation of integrated provirus requires histone acetyltransferase. p300 and P/CAF are coactivators for HIV-1 Tat.". J. Biol. Chem. 273 (38): 24898–905. doi:10.1074/jbc.273.38.24898. PMID 9733796.  

This article incorporates text from the United States National Library of Medicine, which is in the public domain.

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