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Phi X 174: Wikis

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Structure of phage phi X 174 capsid
Phi X 174
Virus classification
Group: Group II (ssDNA)
Family: Microviridae
Genus: Microvirus
Species: phi X 174 phage

The phi X 174 (or phi X) bacteriophage was the first DNA-based genome to be sequenced. This work was completed by Fred Sanger and his team in 1977.[1] In 1962, Walter Fiers had already demonstrated the physical, covalently closed circularity of phi X 174 DNA.[2]

This phage has a very small amount of DNA. Phi X has 11 genes in 5386 bases (it is single stranded) in a circular topology; several of these genes express similar function in two groups. The GC-content is 44% and 95% of nucleotides are coding genes.

In 2003, it was reported that the whole genome of phi X 174 had been assembled synthetically from scratch.[3]

See also

References

  1. ^ Sanger F, Air GM, Barrell BG, Brown NL, Coulson AR, Fiddes CA, Hutchison CA, Slocombe PM, Smith M (1977-02-24). "Nucleotide sequence of bacteriophage phi X174 DNA". Nature 265 (5596): 687–695.  
  2. ^ Fiers W, Sinsheimer RL (1962). "The structure of the DNA of bacteriophage PhiX 174. III. Ultracentrifuge evidence for a ring structure". J. Mol. Biol. 5: 424–434.  
  3. ^ Smith, Hamilton O.; Clyde A. Hutchison, Cynthia Pfannkoch, J. Craig Venter (2003-12-23). "Generating a synthetic genome by whole genome assembly: {phi}X174 bacteriophage from synthetic oligonucleotides". Proceedings of the National Academy of Sciences 100 (26): 15440–15445. doi:10.1073/pnas.2237126100. http://www.pnas.org/cgi/content/abstract/100/26/15440. Retrieved 2007-10-08.  

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