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The rank product is a biologically motivated test for the detection of differentially expressed genes in replicated microarray experiments. It is a simple non-parametric statistical method based on ranks of fold changes. In addition to its use in expression profiling, it can be used to combine ranked lists in various application domains, including proteomics, metabolomics, statistical meta-analysis, and general feature selection.

Calculation of the rank product

Given n genes and k replicates, let eg,i be the fold change and rg,i the rank of gene g in the i-th replicate.

Compute the rank product via the geometric mean: RP(g)=(\Pi_{i=1}^kr_{g,i})^{1/k}

Filled circles represent ranks of one gene in the different replicates. The rank product for this gene would be (2×1×4×2)1/4 ≈ 2

Determination of significance levels

Simple permutation-based estimation is used to determine how likely a given RP value or better is observed in a random experiment.
1. step: generate p permutations of k rank lists of length n
2. step: calculate the rank products of the n genes in the p permutations
3. step: count how many times the rank products of the genes in the permutations are smaller or equal to the observed rank product. Set c to this value.
4. step: calculate the average expected value for the rank product by ERP(g) = c / p
5. step: calculate the percentage of false positives as pfp(g) = ERP(g) / rank(g)
where rank(g) is the rank of gene g in a list of all n genes sorted by increasing RP

References

  • Breitling, R., Armengaud, P., Amtmann, A., and Herzyk, P.(2004) Rank Products: A simple, yet powerful, new method to detect differentially regulated genes in replicated microarray experiments, FEBS Letters, 573:83–-92

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