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Shigella dysenteriae: Wikis

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Shigella dysenteriaedfgjsdkl;fgsdjakl;r
Scientific classification
Kingdom: Bacteria
Phylum: Proteobacteria
Class: Gamma Proteobacteria
Order: Enterobacteriales
Family: Enterobacteriaceae
Genus: Shigella
Species: S. dysenteriae
Binomial name
Shigella dysenteriae
(Shiga 1897)
Castellani & Chalmers 1919

Shigella dysenteriae is a species of the rod-shaped bacterial genus Shigella.[1] Shigella can cause shigellosis (bacillary dysentery). Shigellae are Gram-negative, non-spore-forming, facultatively anaerobic, non-motile bacteria.[2]

S. dysenteriae, spread by contaminated water and food, causes the most severe dysentery because of its potent and deadly Shiga toxin, but other species may also be dysentery agents.[3] Contamination is often caused by bacteria on unwashed hands during food preparation.

Diagnosis

A stool specimen is Gram-stained to show Gram-negative rods, with no particular arrangement. Enrichment is performed by growing the organisms on Selenite-F broth. Then, since the specimen is not sterile, the use of selective plates is mandatory. XLD agar, DCA agar, or HE agar are inoculated and colonies are colorless on all of them as the organism is non-lactose a fermentor. Inoculation of a TSI slant shows an alkaline slant and acidic butt with no gas or H2S production. Following incubation on SIM, the culture appears non-motile with no H2S production. Addition of Kovac's reagent to the SIM tube following growth typically indicates no indole formation (serotypes 2, 7 and 8 produce indole[4]).

It's noteworthy that Shigella flexneri will produce acid and gas from glucose, and Shigella sonnei is mannitol and ornithine positive, and is also late lactose fermentor (ONPG positive). Some Shigella species are capable of producing indole.

References

  1. ^ Ryan KJ, Ray CG (editors) (2004). Sherris Medical Microbiology (4th ed.). McGraw Hill. ISBN 0838585299.  
  2. ^ Hale TL, Keusch GT (1996). Shigella. In: Baron's Medical Microbiology (Baron S et al., eds.) (4th ed.). Univ of Texas Medical Branch. ISBN 0-9631172-1-1. http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mmed.chapter.1257.  
  3. ^ Herold S, Karch H, Schmidt H (2004). "Shiga toxin-encoding bacteriophages--genomes in motion". Int. J. Med. Microbiol. 294 (2-3): 115–21. doi:10.1016/j.ijmm.2004.06.023. PMID 15493821.  
  4. ^ Germani, Y.; Sansonetti, P.J. (2006). "Chapter 3.3.6: The Genus Shigella". in Dworkin, M. (editor-in-chief). The Prokaryotes: Proteobacteria: gamma subclass. Volume 6 (3rd ed.). New York: Springer. pp. 99–122. doi:10.1007/0-387-30746-x_6. ISBN 038725496X.  
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Wikispecies

Up to date as of January 23, 2010

From Wikispecies

Taxonavigation

Main Page
Superregnum: Bacteria
Regnum: Bacteria
Phylum: Proteobacteria
Classis: Gamma Proteobacteria
Ordo: Enterobacteriales
Familia: Enterobacteriaceae
Genus: Shigella
Strain: Shigella dysenteriae
Strain: Shigella dysenteriae Sd197 -

References


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